Selby, the WBC British, Commonwealth and International Featherweight Champion, will square off with Australia's Joel Brunker at London's o2 Arena on Saturday night.
The winner will then advance to challenge the undefeated Russian Evgeny Gradovich for the IBF World Featherweight Championship.
With Brunker unbeaten in his last 27 fights, the toughest challenge of Selby's six-year, 20-match professional career potentially awaits.
However, Selby has thrived in high-pressure scenarios; and already holds an impressive victory over Ryan Walsh at the cavernous Greenwich venue.
Selby vs Brunker will be a headline attraction of Sky Sports 1's Live Fight Night, from 8.30pm this Saturday.