2016-17 finalists Barry Town United will entertain Afan Lido of Welsh League Division One in the second stage of this season's Nathaniel MG Cup.
The boys from Jenner Park received a bye to October's regionalised round of sixteen, as one of four previous semi-finalists (alongside Carmarthen Town, Connah's Quay Nomads and holders The New Saints).
Three JD Welsh Premier League sides were eliminated in Tuesday's opening fixtures, with Bala beaten 1-0 at home by Newtown, Llandundno losing by the same scoreline to Caernarfon and Druids edged out by Airbus in Broughton (AET).
Huws Gray Alliance hopefuls Holywell then pipped WPL newcomers Prestatyn Town in a penalty shout-out on Wednesday night to determine the final entrant into today's draw.
Held at the Vale Resort, the draw for the second round of the Nathaniel MG Cup was made by Wales internationals Joe Allen and Neil Taylor, who selected out the home and away teams, respectively.
The full set of ties for this year's last sixteen reads as follows:
Aberystwyth Town vs Airbus UK Broughton
Newtown vs Holywell Town
Connah's Quay Nomads vs Bangor City
Flint Town United vs The New Saints
Holyhead Hotspur vs Caernarfon Town
Barry Town United vs Afan Lido
Cardiff Met vs Pontypridd Town
Haverfordwest County vs Carmarthen Town
Ties will be played in the week commencing Monday 2nd October, with the Barry/Lido encounter set to be contested on Tuesday 3rd October, under the Jenner Park floodlights.
On their most recent meeting in the competition, played at the same stage in October 1999, Jody Jenkins and Darren Davies netted for Barry in a 2-0 win on Princess Margaret Way.
Lido are three-time holders of the national trophy, securing the silverware in each of its first two seasons (1992-93/1993-94) and then again in 2012. Barry meanwhile have lifted the cup on four successive occasions, from 1997 to 2000.
Updated 12:30 - 31 Oct 2017 by Ashley Cox