Berkshire face a crunch clash with unbeaten Oxfordshire in their final Unicorns Tro phy Group 4 match at Henley's Matson Drive ground on Sunday (11am).
"I can't wait for this one; it's a massive game," said head coach Tom Lambert. "We are well placed in the group and we can win the title. We know what we have do."
Oxon, last year's losing finalists, are two points ahead of Berkshire, so a fourth-straight win will give them top spot and a home tie in the quarter-finals on June 14.
A Berkshire victory will also leave them with six points and, if Herefordshire beat Staffordshire this weekend, then three teams will finish level on points.
Even if they lose, Berks could still finish runners-up in the group, but for that to happen they will need Herefordshire to lose to Staffs at Himley.
The top two in the group qualify for the last eight of this national one-day competition. So, with several permutations, it is likely that the final placings will be decided on run rate.
But Berkshire's Director of Cricket Danny Hall commented: "I think the team need to forget about run rate initially and just concentrate on winning the match.
"Oxfordshire are obviously a very good one-day side, having reached the final last season. They have a 100% record this season. It will be a tough challenge for Berkshire, but we have played very well in our last two matches.
"It would be a great if we can win and go onto the quarter-finals. That would also give the team a massive confidence boost for the remainder of the season."
Experienced all-rounder Steve Naylor, who was not available for the match against Staffs, returns to the side in good form. He took 2-33 and scored an unbeaten 41 for Datchet in their six-wicket win over Amersham in their Homes Counties Premier League Division 2 opener last Saturday.
Five of the 12-man squad are Henley players, while Naylor and James Morris have previously played for the Thames-side club.
Berkshire squad to face Oxon: Michael Roberts (Henley), Waqas Hussain (Slough), Harry Stephens (Datchet), Richard Morris (Bashley), James Morris (capt, Bashley), Steve Naylor (Datchet), Stewart Davison (wktkpr, Henley), Chris Peploe (Ealing), Euan Brock (Henley), Matt Carter (Henley), Tom Nugent (Henley), Callum Gregory (Finchampstead).