Stand-in hooker Dave Beddows and scrum half Dan Harvey scored two tries apiece with further scores from Chris Young, Damian Tierney, Simon Quinn, Chris Pape and Mark Hanson.
Prop forward Young gave Chester the perfect start as he dived over from close range for the first try of the game on three minutes.
Latchford hit back almost immediately but, with both sides showing some slack defence, centre Damien Tierney put Gladiators back in front on seven minutes and the try-fest continued as Latchford hit back on 10 minutes.
Both sides eventually managed to tighten up defensively, although only temporarily as fullback Quinn powered over in the corner on 20 minutes to put Chester back in front.
Beddows then pounced on a loose ball and raced 80 metres for his first try of the afternoon to move the Gladiators further in front.
However, the Warrington-based visitors recovered well and crossed for two tries in four minutes to level up the scores once again.
With half time approaching, stand off Pape took a short ball and barged over the line to send Chester into the break leading 24-20.
Gladiators came out strongly in the second half and were intent on defending much better than in the opening 40 minutes.
Beddows scampered over for his second try of the match in the 45th minute and Dan Harvey added another try 10 minutes later to move Chester 14 points clear.
Latchford regrouped and enjoyed some good possession but were unable to find any chinks in the Gladiators defensive line, with both Quinn and winger Terry Toland producing some outstanding tackles.
Having survived some heavy pressure, Chester then moved 20 points in front as winger Mark Hanson raced away for a try under the posts.
Once again Latchford came back strongly and looked certain to score but Harvey swooped to intercept a long pass and outsprinted the chasing defenders for his second try of the game.
Latchford did eventually manage to break through the Gladiators defence on 76 minutes for a consolation try but Chester were deserved winners following an outstanding second half display.
Chester are at home to Bolton Mets on Saturday in a game which kicks off at 2.30pm at The Cheshire County Sports Club.